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MaleCNS — Drosophila central nervous system connectome (~177k neurons) 3D

eFIB-SEM connectomics (8 nm isotropic) · Drosophila melanogaster (male) · Whole CNS (brain + optic lobes + VNC)

MaleCNS — Drosophila central nervous system connectome (~177k neurons) Open in viewer ▶

⚠ Very large dataset (~176,570 neurons). Please read the viewer guide first — view the whole set zoomed out, then enable Overview before zooming into detail.

MaleCNS is the first finished connectome of an ENTIRE adult male Drosophila melanogaster central nervous system — central brain + optic lobes + ventral nerve cord (VNC) in a single specimen (Berg, Beckett, Costa, Schlegel et al. 2025), imaged by enhanced FIB-SEM at 8 nm isotropic. This scene is the WHOLE connectome: 176,570 proofread neurons across every super class — the optic-lobe and central-brain intrinsic neurons, the descending/ascending bridge between brain and VNC, the sensory and motor periphery, and the VNC intrinsic circuits. It is the male counterpart to the catalogue's BANC (female whole-CNS) connectome. Each neuron is a meshopt-q14-compressed 3D-Tiles surface tagged with the Berg et al. 2025 annotations: super class, class & cell type, soma side and neuromere, hemilineage, predicted neurotransmitter, FlyWire/hemibrain/MANC cross-dataset type matches, and — uniquely for the male CNS — the sexual-dimorphism call (male-specific / sexually dimorphic). Each neuron is colored individually; switch "Color By" to Super Class, Soma Neuromere, Predicted NT, or Dimorphism. At ~177k neurons it is a dense whole-CNS volume — use the viewer's filter / Force-Zoom coarse LOD to explore regions and subsets.

Metadata

features176,570 meshes
zoom0–4
tiles1,217

Provenance & reproducibility

Source: https://www.janelia.org/project-team/flyem/male-cns-connectome
License: CC BY 4.0
Cite: Berg S, Beckett IR, Costa M, Schlegel P, Januszewski M, Marin EC, Nern A, Preibisch S, Qiu W, Takemura S, et al. Sexual dimorphism in the complete connectome of the Drosophila male central nervous system. bioRxiv (2025) doi:10.1101/2025.10.09.680999. Janelia FlyEM MaleCNS v0.9, CC BY 4.0.

download_malecns.py --download --tile --select all --max-neurons 200000 --lod 1 --max-zoom 4 --compression meshopt-q14 (176,570 of 176,571 proofread Neurons; meshes via cloud-volume from the anonymous public bucket precomputed://gs://flyem-male-cns/v0.9/segmentation at multires LOD 1; metadata via neuPrint male-cns:v0.9 custom-Cypher, all Neuron nodes ORDER BY size DESC; u16-quantized meshopt GLB 3D-Tiles octree)
muDM: neuron surfaces → TIN tiles; per-neuron color by bodyId (hash); tags from the Berg et al. 2025 neuPrint annotations: cell_type, super_class, neuron_class, subclass, soma_side, soma_neuromere, hemilineage, predicted_nt, consensus_nt, dimorphism (male-specific/sexually dimorphic), flywire_type / hemibrain_type / manc_type cross-dataset matches, vfb_id, pre/post

Programmatic access

3D Tiles entry point:

https://ngrf.mudm.io/tiles/malecns/tileset.json

Machine descriptors: tilejson.json · croissant.json · full access guide